Analysis on the prokaryotic microbiome in females and embryonic cell cultures of Rhipicephalus sanguineus tropical and temperate lineages from two specific localities in Brazil

Abstract Two lineages of Rhipicephalus sanguineus are known in Brazil: the temperate or southern and tropical northern populations. The distribution patterns both R. have epidemiological implications that can affect vectorial competence concerning Ehrlichia canis, agent canine monocytic ehrlichiosis. Intending to identify microbiomes compare microorganisms sanguineus, we used 16S rRNA (V4-V5 region) gene-based metataxonomic approach, through NGS sequencing on MiSeq Illumina platform. We selected specimens females from environment samples primary embryonic cell cultures, lineages, this was first study investigate prokaryotic microbiome tick cultures. results showed many bacterial taxa detected were typical members host environment. A significant diversity cultures found, with emphasis presence Coxiella all samples, albeit different proportions. species present two ticks may be co-evolved them, thus driving interactions between pathogens they harbor transmit vertebrate hosts.